ADVANCED ORGANIC MATERIALS AND BIOMATERIALS

biotechnologie biomaterialyLaboratory of biotechnology and biomaterials

 

Ferm2

There are many reasons life and living organisms are fascinating. Our laboratory deals primarily with research of the tiniest creatures – microorganisms and their extraordinary properties. These microscopic single-cell organisms often work as perfect chemical factories capable of a wide variety of unique processes. Each and every cell can produce a broad range of various chemicals and biomaterials it needs for its survival. Under certain circumstances, it can even stockpile these products. Our objective is to develop methods and technologies of microbial production of industrial substances such as biomaterials, enzymes, vitamins, pigments and other natural molecules. We also develop ways of using these chemicals, especially in the fields of pharmacy, healthcare, food industry and cosmetics. At the same time, we investigate potential effects of various materials and bioproducts on living cells of all types (e.g. human, microbial, etc.).

Cooperation with industry

Biotechnology and biomaterials lab deals with biotechnologies, applications and characterization of biomaterials. We are fully open to cooperation  with commercial partners both in the analytical support and in the optimazation of the partial process steps as well as in development of innovative concepts and technologies.

For example, we are the author of the patented Hydal Biotechnology (http://hydalbiotech.com) enabling conversion of waste frying oil into bioplastics. The technology was patented in 2013, sold to a commercial partner and a transfer to production is currently under way. This technology and its authoring team have won a number of awards, including the most significant: 

Awards

  • Czech Innovation Award 2013, winner of the category "Innovative Idea" for the team of FCH BUT
  • City of Brno Award for 2015 – Technical Sciences (Prof. Márová)
  • Prestigious international "Frost and Sullivan Technology Innovation Award" for the HYDAL (PHA) technology – so called technology Oscars 2015
  • International European Commission Award "Seal of Excellence" for the project No. 762484, "HYDAL-BIOTECH Biotechnology for the production of PHA biopolymer from waste cooking oil" submitted within H2020 – 2016
  • International recognition – inclusion into the "TOP 10 Products" presented at the prestigious China High Tech Fair 2016 in Shenzen

Selected patents and utility models:

Patents and utility models

  • "Utilization of waste deep fryer oil in the production of microbial bioplastics on the basis of polyhydroxyalkanoates" – registered as Czech patent PS3835CZ on 31 October 2013, license sold to industrial partner and disseminated abroad.
  • "Polymer fibre and the method of preparation thereof" … patented in December 2016 under No. 306448; PCT international patent application filed.
  • "Nanofibre sorptive material made from renewable resources for capturing heavy metals", registered as utility model in July 2016 under No. 30069
  • "Biodegradable fibrous filtration material with sorptive properties" – patent application registered at Industrial Property Office

Overview of laboratory activities which can serve as the basis of cooperation with the industry

  • Determining levels of biologically active substances in various matrixes
  • Determining activity of enzyme and microbiological specimens
  • Determining biological effects
  • Determining antimicrobial activity
  • Determining genotoxicity and cytotoxicity of encapsulation of biologically active substances
  • Optimization of the individual steps of biotechnological processes (up-stream processes, cultivations, down-stream processes)
  • Designing and developing possible strategies for exploitation of waste and by-products of food and other production
  • Preparation of materials on the basis of nano/micro fibres and nano/micro particles for a broad spectrum of applications.

Contractual applied research – Economic cooperation

A well-established and effective platforms for cooperation with the industry is contractual research – i.e. a cooperation on the basis of a specific commission/order of the industrial partner for carrying out previously authorised experiments and consultations, often leading to improving, fixing or optimizing the given technology. Another area of cooperation is determining the level of various types of active substances in natural matrixes. An overview of the main partners can be found below.

Applied research for innovations – Innovation vouchers, GACR, TACR, OP PIK and others

More extensive projects are better implemented by means of aid schemes, in which case our centre usually assumes the role of co-investigator or supplier (in case of innovation vouchers).

Selection of projects implemented in the past 5 years:

Projects

  • TAČR GAMA "VUT Šance", TG01010054, dílčí projekt 1 - "Příprava a využití mikro- a nanovlákenných filtračních materiálů na bázi biopolymeru PHA"(2014-2016); dílčí projekt 2 - "Příprava monomeru a oligomerů 3-hydroxybutyrátu a jejich využití v potravinářství, kosmetice a farmacii" (2016-2018)
  • GAČR - projekt GP15-206455 "Zapojení polyhydroxyalkanoátů ve stresové odezvě bakterií", 2015-2018
  • COST-CZ LD15031 “Využití konceptu biorafinerie k valorizaci odpadní lignocelulózové dřevní biomasy”, 2015-2017
  • TAČR Delta; " Transfer of Biotechnology Hydal to China", TF0200006, 2016-2018
  • MPO – program Aplikace; „Vývoj aplikací PHA na úrovni low-end (zemědělské fólie) i high-end (nanovlákna - filtrace, nanočástice – kosmetika, 3D tisk), 2017-2018
  • Mezinárodní projekt „Lipofungi“ č. 268305, “Bioconversion of low-cost fat materials into high-value PUFA-Carotenoid-rich Biomass, financovaný Research Council of Norway.
  • Inovační vouchery - příklady:
    • Helvetia Pharma – „Identifikace antimikrobiálních vaječných peptidů a ověření jejich antimikrobiálních vlastností ve finálním přípravku – doplňku stravy“, 2013-2014
    • Dekonta – „Studium akumulace PHA u bakteriálních kmenů schopných degradace nafty“, 2014-2015
    • EVECO Brno, spol. s r.o. – „Možnosti neenergetického využití pyrolýzního oleje“, 2016-2017

We currently cooperate with a number of both Czech and foreign companies and institutions. This is a list of our long-term industrial and research partners in the Czech Republic and abroad:

LIST OF COLLABORATING COMPANIES

  • Nafigate Corporation s.r.o., strategic partner – the HYDAL technology and its application
  • Pharmaceutical Biotechnology – characterization of probiotics
  • miniBPS s.r.o.
  • Dekonta, a.s. – biodegradation and decontamination
  • Photo Systems Instruments – optimization of algae cultivation
  • EVECO Brno, spol. s r.o. – biodegradation of wastes
  • Research Institute of Brewing and Malting – separation methods
  • CEITEC, FS MU – genomics and proteomics
  • Institute of Scientific Instruments of the CAS, v.v.i. – spectroscopic and microscopic methods
  • Institute of Microbiology of the CAS, v.v.i.), Prague, Třeboň – pilot cultivation of microorganisms
  • Department of Biotechnology, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava – semi-dry cultivations, analytical methods
  • Institute of Chemistry, Slovak Academy of Sciences, Bratislava, SR – taxonomy of microorganisms
  • NMBU As, Norway, Norilia Company, Norway – exploitation of animal waste by means of microorganisms
  • University of Sassari, Italy – enzyme technologies
  • University of Huelva, Spain – metabolism and utilization of algae, instrumental methods
  • TU Graz, Austria – utilization of waste in PHA production

Professional courses and seminars

Certified course of sensoric analysis of food in accordance with ČSN (Czech National Standard) ISO 8589 for the analysis of food by means of human senses.

SCIENCE AND RESEARCH

Science and research indisputably form the core of the laboratory's activity and represent an endless source of inspiration and knowledge which we strive to use both within the cooperation with our industrial partners and within our project and teaching activities. The basic strategy of our research activity can be divided into several fields:

  • Microbial biotechnology – cultivation, growth and production characteristics of microorganisms
  • Isolation, purification and characterization of biotechnological products and their properties
  • Processing and subsequent utilization of natural molecules and biomaterials for applications in the food industry, cosmetics, pharmaceutics and other fields

Some of the specific scientific topics dealt with in our laboratory are:

  • Biotechnological production of bioplastics on the basis of polyhydroxyalkanoates, enzymes, provitamins, carotenoids and other metabolites including enriched microbial biomass.
  • Valorization of food industry waste substrates, agricultural and other productions.
  • Purification, extraction and isolation of biomolecules and biopolymers from natural materials, molecular characterization of these molecules by means of advanced instrumental techniques
  • Cultivation of photosynthetic microorganisms – algae and cyanobactreriae for the production of healthy biomass
  • Engaging selected metabolites in the stress response of microorganisms
  • Physiology of microorganisms and its regulation
  • Monitoring and optimization of biotechnological processes, transfer to industrial scale (up-stream)
  • Utilization of advanced techniques to analyse viability of microbial cells
  • Preparation, characterization and application of micro/nano fibres and micro/nano particles from biomaterials and utilization of these products
  • Utilization of microorganisms in biodegradation processes
  • Determining antimicrobial effects of natural substances on tested microorganisms
  • Determining potential toxic effects of natural substances and nanomaterials on human cells (cytotoxicity) and cell systems (DNA – genotoxicity) in order to assess the safety of their use

An integral part of the laboratory's activities is supervision of bachelor's, diploma and dissertation theses. Our laboratory is currently supervising around 50 theses.

RESEARCH TEAM

prof. RNDr. Ivana Márová, CSc.

prof. RNDr. Ivana Márová, CSc.

vedoucí laboratoře, biotechnologie, nanotechnologie
(head of laboratory
, biotechnology, nanotechnology)


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 351

doc. Ing. Stanislav Obruča, Ph.D.

doc. Ing. Stanislav Obruča, Ph.D.

výzkumník
(senior researcher)

klíčový pracovník - biotechnologie, technologie bioplastů PHA
(key worker – biotechnology, PHA bioplastics technology)


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 486

Ing. Andrea Hároniková, Ph.D.

Ing. Andrea Hároniková, Ph.D.

výzkumník
(senior researcher)

klíčový pracovník - biotechnologie, kosmetologie
(key worker – biotechnology, cosmetology)


This email address is being protected from spambots. You need JavaScript enabled to view it.

Ing. Eva Vítová, Ph.D.

Ing. Eva Vítová, Ph.D.

výzkumník
(researcher)

odborný pracovník - instrumentální metody, senzorická analýza
(specialist – instrumental methods, sensoric analysis)


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 353

Ing. Petra Matoušková, Ph.D.

Ing. Petra Matoušková, Ph.D.

výzkumník
(junior researcher)

klíčový pracovník - nanotechnologie, buněčné technologie, biotechnologie
(key worker – nanotechnology, cellular technologies, biotechnology)


This email address is being protected from spambots. You need JavaScript enabled to view it.

Mgr. Vojtěch Kundrát

Mgr. Vojtěch Kundrát

technik
(technician)

odborný pracovník - nanotechnologie
(specialist – nanotechnology)


This email address is being protected from spambots. You need JavaScript enabled to view it.

Ing. Martin Szotkowski

Ing. Martin Szotkowski

technik
(technician)

odborný pracovník - nanotechnologie
(specialist – nanotechnology)


This email address is being protected from spambots. You need JavaScript enabled to view it.

Contact person

prof. RNDr. Ivana Márová, CSc.

prof. RNDr. Ivana Márová, CSc.

Vedoucí laboratoře
(head of laboratory)


This email address is being protected from spambots. You need JavaScript enabled to view it.

TEL +420 541 149 351

Contacts

Mgr. Martin Bartoš

obchodní manažer centra
(business Manager)


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 446

Mgr. Lucie Hrbková

finanční manažerka
(financial Manager )


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 482

Mgr. Zuzana Burešová

projektová manažerka
(project Manager )


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 814

Dagmar Terichová

kancelář
(office)


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 813

Marta Horáčková, DiS.

personalistka
(HR Specialist )


This email address is being protected from spambots. You need JavaScript enabled to view it.
TEL +420 541 149 314