ADVANCED ORGANIC MATERIALS AND BIOMATERIALS

laborator biokoloidyLaboratoty of biocolloids

 

laborator biokoloidy

The laboratory of biocolloids does both basic and applied physico-chemical research with emphasis on colloids, especially dispersions and hydrogels of both natural and synthetic origin. We provide our partners with physico-chemical support of their research or materials development. The laboratory has state-of-the-art equipment for fluorescence spectroscopy, thermal analysis and calorimetry, characterization of liquid and solid colloids including their flow properties, or the study of diffusion. The potential applications include the fields of medicine, pharmacy, cosmetics, household and consumer chemicals, nanotechnologies, environmental protection and agriculture.

Collaboration with industry

We provide for our industrial partners with physical and chemical support in their material development, application and product characterization. We are experts in colloids, dispersions, but our techniques and experience have a more universal use.

  • Amagro – new application forms of humic substances
  • Precheza – dispersion for cosmetic applications
  • TernoColor - characterization of ecological colors
  • DCT - Analytical Control of Cleaning Technology
  • TON - development of humidity sensors

INSTRUMENTAL EQUIPMENT OF THE LABORATORY

  • CHARACTERIZATION OF COLLOIDS

    Laboratory of biocolloids possesses expertise and facilities for standard and advanced structural and chemical analysis of colloidal systems as well as stability issues.

    • UV-VIS spektrophotometers Hitachi U3900 and Varian Cary 50

      HITACHI U3300 Cary50

    • FT–IR spektrophotometer Nicolet iS50 including ATR one-time extension and Nicolet iS5

    iS5 iS50

    • optical fiber spectrometer OceanOptics USB2000+ with immersion probes 
      (UV-VIS and fluorescence)
       

    USB2000 fiber probe

    • automatic titrator Schott Titroline Alpha, electrochemical equipment 
      (flow coulometry analyzer Istran EcaFlow 150GLP + standard pH, conductivity and ion-meters)

      titrator EcaFlow electrochemistry meters

    • dispersion analyzer LUMiSizer, Zetasizer Nano ZS
      ultrasound spectrometer 
      HR-US 102T

    LUMiisizer ZetaSizer 2 HRUS 1 

    • SEC Chromatograf (Agilent Technologies) + Optilab T-rex (Wyatt) + Viscostar-II Viscometer (Wyatt) + DAWN HELIOS5-II (Wyatt)

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    • EURO EA Elemental Analyzer (Euro Vector Instruments & Software)

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  • PARTICLE SIZE AND CHARGE ANALYSIS

    Distribution of particle sizes in liquid colloids is analyzed by dynamic light scattering. This instrument enables also measurement of electrokinetic (zeta) potential and its dependence on pH as well as estimate of molecular weight by static light scattering. The zeta potential can be also used for quantification of the stability of colloidal systems. Additionally, particle sizes can be determined by centrifugal dispersion analysis (LUMiSizer) which further enables much broader characterization of colloids, e.g. their stability or the  determination of the rate of sedimentation/creaming. 

    • Zetasizer Nano ZS
    • LUMiSizer

    ZetaSizer 1 LUMiSizer 2

  • FLUORESCENCE SPECTROSCOPY

    Laboratory of biocolloids has unique and universal fluorescence facility. Fluorescence spectroscopy is used to study any system, liquid or solid including powders, which exhibits fluorescence, phosphorescence, chemiluminiscence and other types of luminescence. These include samples of importance in biological and material sciences, systems exhibiting fluorescence on their own as well as systems with added fluorophores. Emission, excitation, and synchronous 3D spectra can be obtained or absolute quantum yields can be measured. Furthermore, we determine fluorescence lifetimes in the range from hundreds of picosecond to tens of seconds. Measurement in polarized light and under different temperatures – from -10 ° C to 100 ° C and at a temperature of liquid nitrogen – is allowed as well. Quantum dots can be measured easily. USB spectrometer in combination with fiber fluorescence probe allows to detect fluorescence emission from illuminated liquids or solids. Fiber probe enable measuring in different depths of sample.

    • Fluorolog (steady-state, polarised fluorescence), FluoroCube (time-resolved fluorescence)
    • AMINCO-Bowman Series 2 (steady-state fluorescence) 
    • Integrating sphere (determination of absolut quantum yields)
    • USB spectrometer OceanOptics USB2000+ with fluorescence probe

    FluoroCube FluoroLog

    AmicoBowman

    IntegratingSphere

  • FLUORESCENCE CORRELATION SPECTROSCOPY

    FCS is based on monitoring fluctuations of the fluorescence intensity originating from species diffusing through a very small observation volume. A correlation analysis of these fluctuations yields information on the diffusion coefficients of the species (rotational and translational), their fluorescence (molecular) brightness, concentration, etc. The temporal fluctuations in the detected fluorescence intensity, caused by fluorescent species diffusing through the observation volume, are recorded and analyzed by an autocorrelation function (τ). Obtained diffusion time τD is related to their diffusion coefficient D. In diluted system the Stokes-Einstein relation can be used to obtain hydrodynamic radius.

    FLUORESCENCE LIFETIME CORRELATION SPECTROSCOPY

    The autocorrelation function in this case (ACF) is calculated from the various arrival times of detected fluorescence photons, either by a hardware autocorrelator or by software processing of the record of arrival times. If the sample contains, for example, two kinds of diffusing fluorophores, both contribute to the detected intensity fluctuations and the resulting ACF is a complicated superposition, which can be evaluated with this method.

    DUAL-FOCUS FLUORESCENCE CORRELATION SPECTROSCOPY

    2fFCS measures absolute values of diffusion coefficients without referencing against a sample with known diffusion coefficient, like it is often done in conventional FCS. The core idea consists of two fundamental changes compared to conventional (single-focus) FCS. At first, the introduction of an external ruler into the measurement system by generating two laterally shifted but overlapping laser foci at a fixed and known distance. At second, these two foci and corresponding detection regions are generated in a way that the corresponding MDFs (molecule detection function) are sufficiently well described by a simple two-parameter model yielding accurate diffusion coefficients.

    General schematic picture

    FCS

    Schematic representation of Fluorescence correlation spectroscopy. a) epifluorescence microscope with temporal fluctuation of fluorescence intensity. b) laser beam shape with schematically sketched confocal volume. c) labeled polymeric sample with fluorophores.

    HARDWARE SETUP

    This equipment is based on MicroTime 200 system (PicoQuant GmbH). This system contains epifluorescence microscope with dry, water and oil immersion objectives (Olympus), four avalanche photodiodes (2x τ-SPAD, 2x MPD) for different spectral ranges, spectrograph and ccd camera (Andor). As excitation sources picosecond laser heads are used with excitation wavelengths 375, 405, 440, 470, 510, and 640 nm. Next excitation option an infrared femtosecond laser can be used for two photon excitation at 1030 nm (Menlo). Because three of laser heads are doubled with different polarization (470, 510, and 640 nm), at these wavelengths dualfocus-FCS measurements can be done.

    • Fluorescence correlation spectroscopy (MicroTime 200, PicoQuant GmbH)

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  • HIGH RESOLUTION ULTRASOUND SPECTROSCOPY

    Ultrasound spectrometer with high resolution is universal equipment for characterization of liquid and semi-solid colloids and solutions. It is used e.g. for study of aggregation processes, intermolecular interactions, colloidal stability, structural characterization of colloids, kinetic of processes ocurring in colloids or of chemical reactions, determination of compressibilities. Measurements can be done also in titration or temperature ramp mode. Examples of use in biotechnology, pharmaceuticals, food, cosmetics, petrochemicals and other industries can be found here. Ultrasound measurements are supported by high precision densitometer which combines accurate automatic measurement of density of liquids and measurement of the sound velocity at lower resolution; both quantities can be measured in dependence on temperature.

    • spectrometer HR-US 102T
    • densitometer DSA 5000

    HRUS 2 densitometer

  • THERMAL ANALYSIS AND CALORIMETRY

    Laboratory of biocolloids is specialized on two methods of thermal analysis – thermogravimery (TG) and differential scanning calorimetry (DSC) – and on modular microcalorimetry. TG and DSC are standard methods to study thermal properties of materials as phase transitions, thermal or thermooxidative stability, compatibility of mixtures etc. Modular calorimeter is highly sensitive and precise instrument that enables to study variety of slow processes accompanied by heat exchange ranging from processes occurring in cells or living organisms through aggregation processes, slow chemical reactions, delicate phase transitions or polymorphisms up to battery discharging or explosive stability. It can be used in isothermal titration mode, as a solution calorimeter (study of heats of dissolution) or in a perfusion arrangement (to study interactions with water vapor, hydration).

    • thermogravimetric analyzers TGA Q5000 and TGA Q50
    • differential scanning calorimeter DSC Q200
    • modular titration microcalorimeter TAM III

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    TAM3

  • RHEOLOGY

    BIOCOL offers relatively a wide range of mechanical analyses of liquid, semisolid, and viscoelastic materials. Characteristics such as the viscosity of fluids, flow properties, yield stress, relaxation properties, viscoelasticity, etc. are measured. 

    • Rheometer ARG2
    • Cappilary microviscosimeter AMVn and digital densitometer DMA 4500

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    The equipment provides measurements of viscosity under either a constant strain, or alternating strain (flow curves), the determination of the yield stress, dynamic viscoelastic properties, the gelation point etc. It allows the evaluation of time stability, ageing of a material, as well as the temperature influence on its mechanical properties and relaxation after a defined strain. The materials that can be measured span various kinds of liquid, biological samples (cell serum, DNA), foods, cosmetics (emulsions, gels, lotions), and pharmaceuticals to construction materials, polymers, etc.

  • MICRORHEOLOGY

    This technique is used for determination of the viscosity and visco-elastic properties of liquids in ultrasmall volumes (typically microliters). Basis of this method is observation of Brownian motion of dispersed particles with defined diameter and subsequent video analysis.

    • Microscope Nikon Eclipse e200

    uRh EN

    • Microscope Nikon Eclipse e200

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  • TENSIOMETRY

    Static surface or interfacial tension is measured by the standard method of platinum plate or ring. Additionally, it is possible to measure the dynamic contact angle, the density of the sample using buyoancy, absorption of a liquid by a powder material and automatically, with utilization of an automatic titrator, also the value of thecritical micellar concentration of surfactants. The method of maximum pressure in the bubble is used to determine of dynamic surface tension (surfaces with lifetime below about 1 s).

    • Tensiometer KSV Sigma 701
    • Bubble tensiometer BPA 800P

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  • EXPERIMENTAL DIFFUSION TECHNIQUES

    Laboratory equipment allows the determination of permeability of various substances through the layers of materials, gels, membranes, etc. According to the nature of the investigated compounds, we apply various analytical methods in order to determine concentration of diffusing compounds such as potentiometry (ISEelectrodes), conductometry, UV-VIS spectroscopy, chronopotenciometry and more. Our laboratory is equipped with a wide range of quality equipment suitable for this use. Model examples of the use are penetration of substances through membranes, permeability of filter and packaging materials, transport of active ingredientsin ophthalmic lenses or drug transport in gels.

    • water-jacketed horizontal diffusion cells
    • optical fiber spectrometer
    • module for measurement of UV-VIS absorption at different positions of the sample

    DiffusionCells DiffusionCells 2

    DiffusionCells 3 diffusion 2

SCIENCE AND RESEARCH

RESEARCH TEAM

prof. Ing. Martina Klučáková, Ph.D.

prof. Ing. Martina Klučáková, Ph.D.

vedoucí laboratoře
(head of laboratory)


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TEL +420 541 149 410

prof. Ing. Miloslav Pekař, CSc.

prof. Ing. Miloslav Pekař, CSc.

výzkumník
(senior researcher)


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TEL +420 541 149 330

Ing. Petr Sedláček, Ph.D.

Ing. Petr Sedláček, Ph.D.

výzkumník
(junior researcher)


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TEL +420 541 149 486

Ing. Filip Mravec, Ph.D.

Ing. Filip Mravec, Ph.D.

výzkumník
(junior researcher)


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TEL +420 541 149 483

Ing. Jitka Krouská, Ph.D.

Ing. Jitka Krouská, Ph.D.

 výzkumník

(junior researcher)

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TEL +420 541 149 399

Ing. Andrea Kargerová, Ph.D.

Ing. Andrea Kargerová, Ph.D.

výzkumník
(junior researcher)


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TEL +420 541 149 399

Ing. Jiří Smilek, Ph.D.

Ing. Jiří Smilek, Ph.D.

výzkumník
(junior researcher)

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TEL +420 541 149 483

Ing. Vojtěch Enev, Ph.D.

Ing. Vojtěch Enev, Ph.D.

výzkumník
(junior researcher)

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TEL +420 541 149 488

ING. MICHAL KALINA, PH.D.

ING. MICHAL KALINA, PH.D.

technik
(technician)

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TEL +420 541 149 399

Contact Person

prof. Ing. Martina Klučáková, Ph.D.

prof. Ing. Martina Klučáková, Ph.D.

vedoucí laboratoře

(head of laboratory)

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TEL +420 541 149 410

Contacts

Mgr. Martin Bartoš

obchodní manažer centra
(business Manager)


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TEL +420 541 149 446

Mgr. Lucie Hrbková

finanční manažerka
(financial Manager )


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TEL +420 541 149 482

Mgr. Zuzana Burešová

projektová manažerka
(project Manager )


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TEL +420 541 149 814

Dagmar Terichová

kancelář
(office)


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TEL +420 541 149 813

Marta Horáčková, DiS.

personalistka
(HR Specialist )


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TEL +420 541 149 314